The Characterization of leaf extract of Lawsonia inermis L. by GC–MS analysis, and its efficacy on post harvest decaying fungi of Psidium guajava L.
Characterization of leaf extract of Lawsonia inermis L. by GC–MS analysis, and its efficacy on post harvest decaying fungi of Psidium guajava L.
Keywords:
Anti-fungal, Post-harvest, Lawsonia inermis L., Leaf extracts, GCMS analysis.Abstract
Different fungi affect Psidium guajava L. in the post-harvest period, causing considerable economic losses and risks to consumer health due to the mycotoxins that some of these fungi produce. Studies on the efficacy of Lawsonia inermis L. methanolic leaf extract were determined on causative agents of guava post-harvest fruit. Different concentration of Lawsonia inermis L. methanolic leaf extract (25, 50 and 75%) was used. The treatments were laid out in a completely randomized design (CRD) with three replications. F. oxysporum, A. alternata, R. stolanifer, Gloesporium psidii and Phomopsis psiddi were isolated and identified to be responsible for the Guava fruit rots. All concentrations of the tested leaf extract of Lawsonia inermis L. significantly suppressed the mycelial growth of the post-harvest decaying fungi. The effect was proportional to the concentration of the leaf extract and suppression was highest at 75% and lowest at 25% concentration. The study revealed that leaf extract of Lawsonia inermis L. proved to be effective in the control of postharvest rots of Guava and serves as a good option to synthetic agrochemicals which are not eco-friendly and biologically safe. The GCMS analysis study revealed the presence of 11 major bioactive compounds in which the presence of Phenol, Benzoic acid, Phytol, and Squalene are known to have antifungal activities.
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References
Afzal M, Al-Oriquat, Al-Hussan JM, Mohammed N. (2010) Flavone glycosides from Lawsonia inermis. Heterocyclic; 14:1973-6.
Anonymous. (2014). Horticulture Database, National Horticulture Board, Government of India publication, 79-80
Arévalo-Marín E, Casas A, Landrum L, Shock MP, Alvarado-Sizzo H, Ruiz-Sanchez E and Clement CR (2021) The Taming of Psidium guajava: Natural and Cultural History of a Neotropical Fruit. Front. Plant Sci. 12:714763. doi: 10.3389/fpls.2021.714763
Boubaya A, Marzougui N, Ben Yahia L, Ferchichi A. (2011) Chemical diversity analysis of Tunisian Lawsonia inermis L. populations. Afr J Biotechnol; 10:4980-87.
Borade AS, Kale BN, Shete RV. (2011) A phytopharmacological review on Lawsonia inermis (Linn). Int J Pharm Life Sci; 2:536-41.
Chaube, H.S. and Pundhir, (2005) V.S Crop diseases and their Management. Prentice- Hall of India Pvt Ltd, New Delhi. India: 641-642
Choi G.J., Jang K.S., Kim J.S., Lee S.W., Cho J.Y., Cho K.Y., Kim J.C. (2004): In vivo antifungal activities of 57 plant extracts against six plant pathogenic fungi. – Journal of Plant Pathology 20:184–191
Dandekar R, Fegade B, Bhaskar VH (2015) GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves, Journal of Pharmacognosy and Phytochemistry 2015; 4(1): 149-154
Evans WC, Evans D (2009.) Trease GE. Trease and Evans Pharmacognosy, 16th Ed., Edinburgh, UK, Saunders Ltd., Elseviers publication (2009).
Irshad, Z.; Hanif, M.A.; Ayub, M.A.; Jilani, M.I.; Tavallali, V. Guava. (2020) In Medicinal Plants of South Asia, 1st ed.; Muhammad, A.H., Haq, N., Muhammad, M.K., Hugh, J.B., Eds.; Elsevier Ltd.: Cambridge, MA, USA; pp. 341–354.
Jain VC, Shah DP, Sonani NG, Dhakara S, Patel NM. (2010) Pharmacognostic and preliminary phytochemical investigation of Lawsonia inermis L leaf. Rom J Biol Plant Boil; 55:127-33.
Lin CH, Zon WX, Lin H, Tan RX. (2001). Antifungal activity of Artemisia annua endophytic cultures against phytopathogenic fungi. – Journal of Biotechnology 88: 277–282
L.V. Dracheva, E.V. Dorozhko, O.A. Avramchuk, E.I. Korotkova, E.P. Ryzhkova, H. Li, I.V. Danilova (2009), Voltammetric study of the antioxidant activity of propionic acid bacteria in liquid cultures Moscow Uni. Biol. Sci. Bull., 64 (2009), pp. 157-160.
Morton JF (1987). "Guava" Fruits of Warm Climates. Purdue University.pp. 356–363. Retrieved 24 April 2015
Mares D., Tosi B., Poh F., Andreotti E., Romagnoli C. (2004): Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: ultra structural evidence of Pythium ultimum. Microbiol. Research 159: 295–304
Nwinyi, O.C., S.N. Chinedu, O.O. Ajani, C.O. Ikpo and K.O. Ogunniran, (2008) Evaluation of the antibacterial activity of Pisidium guajava and Gongronema latifolium. J Med Plants Res. 2(8):189–92.
Okemo PO, Bais HP, Vivanco JM. (2003): In vitro activities of Maesa lanceolata extracts against fungal plant pathogens. – Fitoterapia 74: 312–316.
Perez-Sanchez, R., Infante, F., Galvez, C., & Ubera, J. L.. (2007): Fungitoxic activity against phytopathogenic fungi and the chemical composition of Thymus zygis essential oil. – Food Science and Technology International 13: 341–347
Raji R., Raveendran K. (2013): Antifungal activity of selected plant extracts against phytopathogenic fungi Aspergillus niger. – Asian Journal of Plant Science and Research 3: 13-15.
Reddy, L.H.; Couvreur, P. (2009) Squalene: A natural triterpene for use in disease management and therapy. Adv. Drug Deliv. Rev. 2009, 61, 1412–1426. [CrossRef] [PubMed].
Redfern J, Kinninmonth M, Burdass D, and Verran J. (2014). Using Soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. Journal of Microbiology and Biology Education; 15 (1): 45-46 (2014).
Sharma RK, Goel A, Bhatia AK. (2016) Antityphoid activity and phytochemical screening of different extracts of L. inermis plant leaves. Int J Curr Res 2016; 8:37539-42.
S.S Gololo1, N.S Mapfumari1, L.S Sethog, M.T Olivier, L.J Shai and M.A Mogale (2016) Identification of Phytochemical Constituents within the N-Hexane Leaf Extract of Senna italica (Mill) using Gas Chromatography-Mass Spectrometry (GC-MS) analysis J. Pharm. Sci. & Res. Vol. 8(10), 2016, 1141-1143
Sofu JN, Beuchat LR, Davidson PM, Johonson I. (1998) Naturally Occurring Antimicrobial in Food. Task Force Report No. 132, Council for Agricultural, Science, and Technology, Ames, Iowa, 1998.
Song F. and Goodman, R.M. (2001). Physiology and Molecular Plant Pathology, 59:1-11.
Singh D., Sharma RR. (2007). Postharvest diseases of fruit and vegetables and their management. In: Prasad, D. (Ed.), Sustainable Pest Management. Daya Publishing House, New Delhi, India.
Yoshihiro, I.; Toshiko, H.; Shiraishi, A.; Hirose, K.; Hamashima, H. (2005) Effects of geranyl geraniol, teprenone and phytol on the growth of Staphylococcus aureus. Antimicrob. Agents Chemother. 2005, 49, 1770–1774.
Yadav P, Harisha CR, Prajapati PK, (2011) Validation of pharmacopoeial characters of Bhringaraja (Eclipta alba Hassk.). Journal of Current Pharmaceutical Research; 8 (1): 17-24 (2011).
Zumrutdal E, Ozaslan M. (2012) A miracle plant for the herbal pharmacy; henna (Lawsonia inermis). Pharmacology; 896:483-9.
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