Antifungal activity and GC-MS detection of Argyreia nervosa L. leaves extract against Pythium ultimum, a pathogen causing damping-off of Broccoli
Keywords:
Argyreia nervosa L, Pythium ultimum, GCMS, Damping-offAbstract
This study investigates the fungitoxic effects of Argyreia nervosa L. on Pythium ultimum Trow., the causal agent of broccoli damping-off. In vitro experiments utilized acetone extracts at concentrations of 5, 10, 15, and 20% resulting in suppressed fungal mycelial growth. Synergistic assessments involving Trichoderma viride, T. harzianum, and Argyreia leaf extract revealed the most significant inhibition of mycelial growth. Compatibility between the antagonists and the extract was established. Analysis of the extract identified 20 compounds, including antifungal elements like n-hexadecanoic and 9, 12-octadecadienoic acid. This study highlights Argyreia nervosa L. fungitoxic potential and its compatibility with biocontrol agents, suggesting its promising role in fungal disease management.
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