Production of L-glutaminase by halotolerant Bacillus subtilis MM1

Authors

  • Mohammed Mujahed Department of Biotechnology, COCSIT, Latur, MS, India.
  • Kareppa BM Department of Biotechnology, D.S.M. College, Parbhani MS, India.

Abstract

In the present study the L-glutaminase producing halotolerant Bacillus subtilis MM1 isolated from marine environment was screened for the production of various industrially important enzymes like lipase, amylase, protease, chitinase, xylanase and cellulase. The optimization of different physicochemical parameters was carried out for the production of L-glutaminase. The Bacillus subtilis MM1 produced amylase, lipase, chitinase and cellulase. The optimum pH, temperature, salt and L-glutamine concentration for production of the L-glutaminase was found to be 8, 35°C, 3% and 1%. The xylose as carbon source and yeast extract as nitrogen source has supported the maximum yield of L-glutaminase. After optimization of different fermentation parameters the yield of L-glutaminase increased from 47±0.43 to 104±0.24 IU/ml.                                                              

Keywords: Bacillus subtilis, L-glutaminase, Lipase, media optimization.

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Published

2020-09-29

How to Cite

Mohammed Mujahed, & Kareppa BM. (2020). Production of L-glutaminase by halotolerant Bacillus subtilis MM1. International Journal of Life Sciences, 71–78. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/124

Issue

Special issue, A14 | June 2020

Section

Special issue

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Copyright (c) 2020 Mohammed Mujahed, Kareppa BM

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