Determination of antioxidant activities of algae from lentic ecosystems under anthropogenic stress: A comparative study
Keywords:
Antioxidant activity, DPPH, Phenolics, Flavonoids, Spirogyra fluviatilisAbstract
The present study aim to investigate/compare the antioxidant activity of different species of algae collected specifically from water bodies under stress. Total phenolics and flavonoid content of the different algal extracts was determined using Folin Cio Calteau reagent and Quercetin as standard. Results indicate that the total phenolics is maximum (48.88 µgGAE/ml of extract) in Spirogyra fluviatilis and minimum (3.06 µgGAE/ml of extract) in Nitzschia amphibia, flavonoids content was highest (34.93 µgQE/ml of extract) in Klebsormidium flaccidum and lowest (4.75 µgQE/ml of extract) in Anabaena azollae. DPPH radical scavenging activity, Superoxide anion radical scavenging activity was performed in order to determine the antioxidant activities of the extracts prepared from the different species of algae. It was found that all the species of algae do possessed antioxidant activities. However, Spirogyra fluviatilis out performed all the other algal species in that it has a better ability to scavenge DPPH radicals, superoxide anion radicals and that it has a better reducing power in all the tested concentrations (500 µg/ml, 250 µg/ml, 125 µg/ml, 62.5 µg/ml and 31.25 µg/ml) of extract compared to the other algal species which indicate that it could therefore serve as a good and reliable source of natural antioxidants.
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