In Vitro anti-fungal activity of Tinospora cordifolia and Adhatoda vasica against post-harvest fungal pathogens in Apple fruits

Authors

  • Baviskar RN Department of Botany, ICLES’ M. J. College, Vashi, Navi Mumbai. (M.S.) India.

Keywords:

Tinospora  cordifolia, Adhatoda vasica, Plant extracts, Post-harvest pathogens, Disease management

Abstract

The main aim of the present study was to find out antifungal activities of natural medicinal herbs on fungal pathogens.  Ethanolic extracts of Tinospora cordifolia and Adhatoda vasica was observed at concentrations of different ranging from 500, 1000, 1500, 2000, 2500 and 3000µg/ml. were tested against dominant fungal pathogens for antifungal activity in vitro on Penicillium expansum, Aspergillus flavus, and Mucor piriformis isolated from naturally infected apple fruit. Both plants material were collected from different places of Maharashtra and identified. Ethanolic extracts of both medicinal herbs were assessed for antifungal susceptibility using dilution method. Known antifungal properties were used as positive control.  The water extracts were used as control and it was observed that the ethanolic extracts concentrations were more effective and showed antifungal activity against the test pathogens. The results are revealed that Tinospora cordifolia showed 99% mycelial growth inhibition at 3000µg/ml against Penicillium expansum, Aspergillus flavus, whereas Adhatoda vasica showed 100% mycelial growth inhibition at 3000µg/ml against Mucor piriformis. Hence, the results of the present investigation indicate that the plant extracts possess antifungal properties that can be exploited as an ideal treatment for future plant disease management to eliminate fungal spread.

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References

Dahiwale MA, Baviskar RN and Suryawanshi NS (2009) Integreted management of carbendazim resistant Altrenaria alternata causing fruit rot of Pomeranate. Life Sci J Bioinfolate 6 (1):44-45.

Datar VV (1988) Antifungal activity of plant extracts against Fusarium Sp. Causing rhizome rot of ginger paper presented in National Seminar on Management of Crop- Diseases with Plant products/biological agent. Agril College and Res Inst Madurai India PP 28.

Francisco DH (2010) Lippia graveolens and Carya illinoensis Organic Extracts and there in vitro Effect Against Rhizoctonia Solani Kuhn. American J of Agricultural and Biological Sci 5 (3): 380-384.

Gangawane LV (2008) Glimses of phytopathology for sustainable Agriculture. Indian Phytopath. 61 (1):2-8.

Janisiewicz WJ and Korsten L (2002) Biocontrol of post harvest diseases of fruits Annu Rev Phytopathology. 40: 411-441.

Khandare KR Waghmare MB Khandhare NK Patil AV and Kamble SS (2007) Effect of biopesticides in combination with systematic and Non systemic fungicides on growth of some pathogenic fungi. Bioinfolet 4(1): 52-56.

Kumar A Shukla R Singh P Prasad CS and Dubey NK (2008) Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Int Food Sci Emerg 4: 575-580.

McOnie KC (1964) The latent occurrence in citrus and other hosts of a Guignardia easily confused with G. citricarpa, the black spot pathogen. Phytopathology 54: 40- 43.

Mossini SAG, C Carla and C Kemmelmeier (2009) Effect of neem leaf extract and Neem oil on Penicillium growth, sporulation, morphology and ochratoxin a production. Toxins 1: 3-13.

Nene YL and Thapiyal PN (1993) Fungicides in plant diseases control, Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi.

Obagwa J Korsten L (2002) Control of citrus and green and blue molds with garlic extracts. Plant Pathology.109,221- 225.

Ruch Ba Worf R (2001) Processing of neem for plant protection simple and sophisticated standardized extracts. Abstracts of the .Work shop Neem and Pheromones University of Uberaba Brazil March 29-30 Augusts, P.499.

Samson JA (1984) Tropical fruits- Tropical agricultural series. Longman Inc., New York pp 64-118.

Shinde SL, Junne SB, Wadje and Baig MMV (2009) The diversity of antibacterial compounds of Terminalia sp. (Combrataceae). Pakistan J of Biological Sci 12 (22): 1483-1486.

Zhang H Zheng X (2005) Biological control of postharvest blue mold of oranges by Cryptococcus laurentii (Kufferath) Skinner. Biocontrol 50: 331-342.

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Published

2021-03-31

How to Cite

Baviskar RN. (2021). In Vitro anti-fungal activity of Tinospora cordifolia and Adhatoda vasica against post-harvest fungal pathogens in Apple fruits. International Journal of Life Sciences, 9(1), 103–106. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/369

Issue

Vol. 9 No. 1 (2021): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2021 Baviskar RN

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