L-Arginine monohydrochloride analysis by Reverse Phase High Performance Liquid Chromatography (RP-HPLC)
Keywords:
Arginine, Reverse Phase High Performance Liquid Chromatography, RP-HPLC, C18 column, Peak Concentration, Retention time, Primaide organizer, Isocratic Pump SystemAbstract
Reverse Phase High Performance Liquid Chromatography (RP-HPLC) has demonstrated to be a decent method for examination of biochemical samples, such as, amino acids. L-Arginine monohydrochloride is an amino acid which seems to be white in colour and is available in crystal in addition to lyophilized powder form. The current examination intends to the analysis of standard L-Arginine monohydrochloride for two different concentrations i.e. 100 and 200 parts per million (ppm). A simple sample preparation strategy was embraced for the analysis. The lyophilized powder of standard amino acid was solubilised in HPLC grade water. The gradient HPLC system was used as an analytical instrument with reverse phase (C18) column and isocratic pump system. The detection of target analyte was done by diode array detector. As a result of the study, the data of standard amino acid (L-Arginine monohydrochloride) in two different concentrations of 100 and 200 ppm were archived in chromatographic reports and in the chromatograms; the Peak Concentration in percentage, Retention time in minutes and Area in milli absorbance units at 100 ppm are 36.659, 3.040, 42915 and at 200 ppm are 93.626, 2.487, 37186 respectively.
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